Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes
Identifieur interne : 000138 ( Main/Exploration ); précédent : 000137; suivant : 000139Multiplex Screening Assay for Identifying Cytotoxic CD8+ T Cell Epitopes
Auteurs : Chek Meng Poh [République populaire de Chine] ; Jian Zheng [États-Unis] ; Rudragouda Channappanavar [États-Unis] ; Zi Wei Chang [Singapour] ; Thi H. O. Nguyen [Australie] ; Laurent Rénia [Singapour] ; Katherine Kedzierska [Australie] ; Stanley Perlman [États-Unis] ; Leo L. M. Poon [République populaire de Chine]Source :
- Frontiers in Immunology [ 1664-3224 ] ; 2020.
Abstract
The cytotoxicity of epitope-specific CD8+ T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8+ T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8+ T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8+ T cells, thereby allowing to measure the actual epitope-specific cytotoxicity activities. This technique is shown to be useful to study both human and mouse CD8+ T cells. Besides, our results from human PBMCs and three independent infectious animal models (MERS, influenza and malaria) further reveal that IFNγ expression by epitope-specific CD8+ T cells does not always correlate with their cell-killing potential, highlighting the need for using cytotoxicity assays in specific contexts (e.g., evaluating vaccine candidates). Overall, our approach opens up new possibilities for comprehensive analyses of CD8+ T cell cytotoxicity in a practical manner.
Url:
DOI: 10.3389/fimmu.2020.00400
PubMed: 32218786
PubMed Central: 7078160
Affiliations:
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O." last="Nguyen">Thi H. O. Nguyen</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne</institution>,<addr-line>Melbourne, VIC</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Renia, Laurent" sort="Renia, Laurent" uniqKey="Renia L" first="Laurent" last="Rénia">Laurent Rénia</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Singapore Immunology Network, Agency of Science, Technology and Research</institution>,<addr-line>Singapore</addr-line>,<country>Singapore</country></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne</institution>,<addr-line>Melbourne, VIC</addr-line>,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa</institution>,<addr-line>Iowa City, IA</addr-line>,<country>United States</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong</institution>,<addr-line>Hong Kong</addr-line>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Immunology</title><idno type="eISSN">1664-3224</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The cytotoxicity of epitope-specific CD8<sup>+</sup> T cells is usually measured indirectly through IFNγ production. Existing assays that directly measure this activity are limited mainly to measurements of up to two specificities in a single reaction. Here, we develop a multiplex cytotoxicity assay that allows direct, simultaneous measurement of up to 23 different specificities of CD8<sup>+</sup> T cells in a single reaction. This can greatly reduce the amount of starting clinical materials for a systematic screening of CD8<sup>+</sup> T cell epitopes. In addition, this greatly enhanced capacity enables the incorporation of irrelevant epitopes for determining the non-specific killing activity of CD8<sup>+</sup> T cells, thereby allowing to measure the actual epitope-specific cytotoxicity activities. This technique is shown to be useful to study both human and mouse CD8<sup>+</sup> T cells. Besides, our results from human PBMCs and three independent infectious animal models (MERS, influenza and malaria) further reveal that IFNγ expression by epitope-specific CD8<sup>+</sup> T cells does not always correlate with their cell-killing potential, highlighting the need for using cytotoxicity assays in specific contexts (e.g., evaluating vaccine candidates). Overall, our approach opens up new possibilities for comprehensive analyses of CD8<sup>+</sup> T cell cytotoxicity in a practical manner.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhang, N" uniqKey="Zhang N">N Zhang</name></author><author><name sortKey="Bevan, Mj" uniqKey="Bevan M">MJ Bevan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Durgeau, A" uniqKey="Durgeau A">A Durgeau</name></author><author><name sortKey="Virk, Y" uniqKey="Virk Y">Y Virk</name></author><author><name sortKey="Corgnac, S" uniqKey="Corgnac S">S Corgnac</name></author><author><name sortKey="Mami Chouaib, F" uniqKey="Mami Chouaib F">F Mami-Chouaib</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Halle, S" uniqKey="Halle S">S Halle</name></author><author><name sortKey="Keyser, Ka" uniqKey="Keyser K">KA Keyser</name></author><author><name sortKey="Stahl, Fr" uniqKey="Stahl F">FR Stahl</name></author><author><name sortKey="Busche, A" uniqKey="Busche A">A Busche</name></author><author><name sortKey="Marquardt, A" uniqKey="Marquardt A">A Marquardt</name></author><author><name sortKey="Zheng, X" uniqKey="Zheng X">X Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Halle, S" uniqKey="Halle S">S Halle</name></author><author><name sortKey="Halle, O" uniqKey="Halle O">O Halle</name></author><author><name sortKey="Forster, R" uniqKey="Forster R">R Forster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez Lostao, L" uniqKey="Martinez Lostao L">L Martinez-Lostao</name></author><author><name sortKey="Anel, A" uniqKey="Anel A">A Anel</name></author><author><name sortKey="Pardo, J" uniqKey="Pardo J">J Pardo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodriguez, F" uniqKey="Rodriguez F">F Rodriguez</name></author><author><name sortKey="Harkins, S" uniqKey="Harkins S">S Harkins</name></author><author><name sortKey="Slifka, Mk" uniqKey="Slifka M">MK Slifka</name></author><author><name sortKey="Whitton, Jl" uniqKey="Whitton J">JL Whitton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, F" uniqKey="Liu F">F Liu</name></author><author><name sortKey="Whitton, Jl" uniqKey="Whitton J">JL Whitton</name></author><author><name sortKey="Slifka, Mk" uniqKey="Slifka M">MK Slifka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Teixeira, Lk" uniqKey="Teixeira L">LK Teixeira</name></author><author><name sortKey="Fonseca, Bp" uniqKey="Fonseca B">BP Fonseca</name></author><author><name sortKey="Vieira De Abreu, A" uniqKey="Vieira De Abreu A">A Vieira-De-Abreu</name></author><author><name sortKey="Barboza, Ba" uniqKey="Barboza B">BA Barboza</name></author><author><name sortKey="Robbs, Bk" uniqKey="Robbs B">BK Robbs</name></author><author><name sortKey="Bozza, Pt" uniqKey="Bozza P">PT Bozza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhat, P" uniqKey="Bhat P">P Bhat</name></author><author><name sortKey="Leggatt, G" uniqKey="Leggatt G">G Leggatt</name></author><author><name sortKey="Waterhouse, N" uniqKey="Waterhouse N">N Waterhouse</name></author><author><name sortKey="Frazer, Ih" uniqKey="Frazer I">IH Frazer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schroder, K" uniqKey="Schroder K">K Schroder</name></author><author><name sortKey="Hertzog, Pj" uniqKey="Hertzog P">PJ Hertzog</name></author><author><name sortKey="Ravasi, T" uniqKey="Ravasi T">T Ravasi</name></author><author><name sortKey="Hume, Da" uniqKey="Hume D">DA Hume</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Badovinac, Vp" uniqKey="Badovinac V">VP Badovinac</name></author><author><name sortKey="Tvinnereim, Ar" uniqKey="Tvinnereim A">AR Tvinnereim</name></author><author><name sortKey="Harty, Jt" uniqKey="Harty J">JT Harty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tewari, K" uniqKey="Tewari K">K Tewari</name></author><author><name sortKey="Nakayama, Y" uniqKey="Nakayama Y">Y Nakayama</name></author><author><name sortKey="Suresh, M" uniqKey="Suresh M">M Suresh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brunner, Kt" uniqKey="Brunner K">KT Brunner</name></author><author><name sortKey="Mauel, J" uniqKey="Mauel J">J Mauel</name></author><author><name sortKey="Cerottini, Jc" uniqKey="Cerottini J">JC Cerottini</name></author><author><name sortKey="Chapuis, B" uniqKey="Chapuis B">B Chapuis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bruning, Jw" uniqKey="Bruning J">JW Bruning</name></author><author><name sortKey="Kardol, Mj" uniqKey="Kardol M">MJ Kardol</name></author><author><name sortKey="Arentzen, R" uniqKey="Arentzen R">R Arentzen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koutsakos, M" uniqKey="Koutsakos M">M Koutsakos</name></author><author><name sortKey="Wheatley, Ak" uniqKey="Wheatley A">AK Wheatley</name></author><author><name sortKey="Loh, L" uniqKey="Loh L">L Loh</name></author><author><name sortKey="Clemens, Eb" uniqKey="Clemens E">EB Clemens</name></author><author><name sortKey="Sant, S" uniqKey="Sant S">S Sant</name></author><author><name sortKey="Nussing, S" uniqKey="Nussing S">S Nussing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author><author><name sortKey="Li, K" uniqKey="Li K">K Li</name></author><author><name sortKey="Wohlford Lenane, C" uniqKey="Wohlford Lenane C">C Wohlford-Lenane</name></author><author><name sortKey="Agnihothram, Ss" uniqKey="Agnihothram S">SS Agnihothram</name></author><author><name sortKey="Fett, C" uniqKey="Fett C">C Fett</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valkenburg, Sa" uniqKey="Valkenburg S">SA Valkenburg</name></author><author><name sortKey="Li, Ot" uniqKey="Li O">OT Li</name></author><author><name sortKey="Mak, Pw" uniqKey="Mak P">PW Mak</name></author><author><name sortKey="Mok, Ck" uniqKey="Mok C">CK Mok</name></author><author><name sortKey="Nicholls, Jm" uniqKey="Nicholls J">JM Nicholls</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Dijk, Mr" uniqKey="Van Dijk M">MR Van Dijk</name></author><author><name sortKey="Waters, Ap" uniqKey="Waters A">AP Waters</name></author><author><name sortKey="Janse, Cj" uniqKey="Janse C">CJ Janse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoeli, M" uniqKey="Yoeli M">M Yoeli</name></author><author><name sortKey="Most, H" uniqKey="Most H">H Most</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malleret, B" uniqKey="Malleret B">B Malleret</name></author><author><name sortKey="Claser, C" uniqKey="Claser C">C Claser</name></author><author><name sortKey="Ong, As" uniqKey="Ong A">AS Ong</name></author><author><name sortKey="Suwanarusk, R" uniqKey="Suwanarusk R">R Suwanarusk</name></author><author><name sortKey="Sriprawat, K" uniqKey="Sriprawat K">K Sriprawat</name></author><author><name sortKey="Howland, Sw" uniqKey="Howland S">SW Howland</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Huckelhoven, A" uniqKey="Huckelhoven A">A Huckelhoven</name></author><author><name sortKey="Hong, J" uniqKey="Hong J">J Hong</name></author><author><name sortKey="Jin, N" uniqKey="Jin N">N Jin</name></author><author><name sortKey="Mani, J" uniqKey="Mani J">J Mani</name></author><author><name sortKey="Chen, Ba" uniqKey="Chen B">BA Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hadrup, Sr" uniqKey="Hadrup S">SR Hadrup</name></author><author><name sortKey="Bakker, Ah" uniqKey="Bakker A">AH Bakker</name></author><author><name sortKey="Shu, Cj" uniqKey="Shu C">CJ Shu</name></author><author><name sortKey="Andersen, Rs" uniqKey="Andersen R">RS Andersen</name></author><author><name sortKey="Van Veluw, J" uniqKey="Van Veluw J">J Van Veluw</name></author><author><name sortKey="Hombrink, P" uniqKey="Hombrink P">P Hombrink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Christen, U" uniqKey="Christen U">U Christen</name></author><author><name sortKey="Darwiche, R" uniqKey="Darwiche R">R Darwiche</name></author><author><name sortKey="Thomas, He" uniqKey="Thomas H">HE Thomas</name></author><author><name sortKey="Wolfe, T" uniqKey="Wolfe T">T Wolfe</name></author><author><name sortKey="Rodrigo, E" uniqKey="Rodrigo E">E Rodrigo</name></author><author><name sortKey="Chervonsky, A" uniqKey="Chervonsky A">A Chervonsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondo, M" uniqKey="Kondo M">M Kondo</name></author><author><name sortKey="Murakawa, Y" uniqKey="Murakawa Y">Y Murakawa</name></author><author><name sortKey="Harashima, N" uniqKey="Harashima N">N Harashima</name></author><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S Kobayashi</name></author><author><name sortKey="Yamaguchi, S" uniqKey="Yamaguchi S">S Yamaguchi</name></author><author><name sortKey="Harada, M" uniqKey="Harada M">M Harada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wueest, S" uniqKey="Wueest S">S Wueest</name></author><author><name sortKey="Rapold, Ra" uniqKey="Rapold R">RA Rapold</name></author><author><name sortKey="Schumann, Dm" uniqKey="Schumann D">DM Schumann</name></author><author><name sortKey="Rytka, Jm" uniqKey="Rytka J">JM Rytka</name></author><author><name sortKey="Schildknecht, A" uniqKey="Schildknecht A">A Schildknecht</name></author><author><name sortKey="Nov, O" uniqKey="Nov O">O Nov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuwano, K" uniqKey="Kuwano K">K Kuwano</name></author><author><name sortKey="Arai, S" uniqKey="Arai S">S Arai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smyth, Mj" uniqKey="Smyth M">MJ Smyth</name></author><author><name sortKey="Krasovskis, E" uniqKey="Krasovskis E">E Krasovskis</name></author><author><name sortKey="Johnstone, Rw" uniqKey="Johnstone R">RW Johnstone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, An" uniqKey="Liu A">AN Liu</name></author><author><name sortKey="Mohammed, Az" uniqKey="Mohammed A">AZ Mohammed</name></author><author><name sortKey="Rice, Wr" uniqKey="Rice W">WR Rice</name></author><author><name sortKey="Fiedeldey, Dt" uniqKey="Fiedeldey D">DT Fiedeldey</name></author><author><name sortKey="Liebermann, Js" uniqKey="Liebermann J">JS Liebermann</name></author><author><name sortKey="Whitsett, Ja" uniqKey="Whitsett J">JA Whitsett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lim, Wc" uniqKey="Lim W">WC Lim</name></author><author><name sortKey="Khan, Am" uniqKey="Khan A">AM Khan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casimiro, Dr" uniqKey="Casimiro D">DR Casimiro</name></author><author><name sortKey="Wang, F" uniqKey="Wang F">F Wang</name></author><author><name sortKey="Schleif, Wa" uniqKey="Schleif W">WA Schleif</name></author><author><name sortKey="Liang, X" uniqKey="Liang X">X Liang</name></author><author><name sortKey="Zhang, Zq" uniqKey="Zhang Z">ZQ Zhang</name></author><author><name sortKey="Tobery, Tw" uniqKey="Tobery T">TW Tobery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pleguezuelos, O" uniqKey="Pleguezuelos O">O Pleguezuelos</name></author><author><name sortKey="Robinson, S" uniqKey="Robinson S">S Robinson</name></author><author><name sortKey="Fernandez, A" uniqKey="Fernandez A">A Fernandez</name></author><author><name sortKey="Stoloff, Ga" uniqKey="Stoloff G">GA Stoloff</name></author><author><name sortKey="Mann, A" uniqKey="Mann A">A Mann</name></author><author><name sortKey="Gilbert, A" uniqKey="Gilbert A">A Gilbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rodrigue Gervais, Ig" uniqKey="Rodrigue Gervais I">IG Rodrigue-Gervais</name></author><author><name sortKey="Labbe, K" uniqKey="Labbe K">K Labbe</name></author><author><name sortKey="Dagenais, M" uniqKey="Dagenais M">M Dagenais</name></author><author><name sortKey="Dupaul Chicoine, J" uniqKey="Dupaul Chicoine J">J Dupaul-Chicoine</name></author><author><name sortKey="Champagne, C" uniqKey="Champagne C">C Champagne</name></author><author><name sortKey="Morizot, A" uniqKey="Morizot A">A Morizot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varadarajan, N" uniqKey="Varadarajan N">N Varadarajan</name></author><author><name sortKey="Julg, B" uniqKey="Julg B">B Julg</name></author><author><name sortKey="Yamanaka, Yj" uniqKey="Yamanaka Y">YJ Yamanaka</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Ogunniyi, Ao" uniqKey="Ogunniyi A">AO Ogunniyi</name></author><author><name sortKey="Mcandrew, E" uniqKey="Mcandrew E">E Mcandrew</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tau, Gz" uniqKey="Tau G">GZ Tau</name></author><author><name sortKey="Cowan, Sn" uniqKey="Cowan S">SN Cowan</name></author><author><name sortKey="Weisburg, J" uniqKey="Weisburg J">J Weisburg</name></author><author><name sortKey="Braunstein, Ns" uniqKey="Braunstein N">NS Braunstein</name></author><author><name sortKey="Rothman, Pb" uniqKey="Rothman P">PB Rothman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Horton, H" uniqKey="Horton H">H Horton</name></author><author><name sortKey="Russell, N" uniqKey="Russell N">N Russell</name></author><author><name sortKey="Moore, E" uniqKey="Moore E">E Moore</name></author><author><name sortKey="Frank, I" uniqKey="Frank I">I Frank</name></author><author><name sortKey="Baydo, R" uniqKey="Baydo R">R Baydo</name></author><author><name sortKey="Havenar Daughton, C" uniqKey="Havenar Daughton C">C Havenar-Daughton</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li><li>République populaire de Chine</li><li>Singapour</li><li>États-Unis</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Poh, Chek Meng" sort="Poh, Chek Meng" uniqKey="Poh C" first="Chek Meng" last="Poh">Chek Meng Poh</name></noRegion><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name></country><country name="États-Unis"><noRegion><name sortKey="Zheng, Jian" sort="Zheng, Jian" uniqKey="Zheng J" first="Jian" last="Zheng">Jian Zheng</name></noRegion><name sortKey="Channappanavar, Rudragouda" sort="Channappanavar, Rudragouda" uniqKey="Channappanavar R" first="Rudragouda" last="Channappanavar">Rudragouda Channappanavar</name><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name></country><country name="Singapour"><noRegion><name sortKey="Chang, Zi Wei" sort="Chang, Zi Wei" uniqKey="Chang Z" first="Zi Wei" last="Chang">Zi Wei Chang</name></noRegion><name sortKey="Renia, Laurent" sort="Renia, Laurent" uniqKey="Renia L" first="Laurent" last="Rénia">Laurent Rénia</name></country><country name="Australie"><noRegion><name sortKey="Nguyen, Thi H O" sort="Nguyen, Thi H O" uniqKey="Nguyen T" first="Thi H. O." last="Nguyen">Thi H. O. Nguyen</name></noRegion><name sortKey="Kedzierska, Katherine" sort="Kedzierska, Katherine" uniqKey="Kedzierska K" first="Katherine" last="Kedzierska">Katherine Kedzierska</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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